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| | |
Numbers of c.f.u.
|
Numbers of endospore
|
|---|
| |
Sampling site
|
NaCl 0%
|
NaCl 20%
|
NaCl 0%
|
NaCl 20%
|
|---|
|
(A)
|
Inland soils
| | | | |
| |
Warabi, S
|
340,000
|
14
|
89,000
|
3
|
| |
Koto, T
|
186,000
|
6
|
11,000
|
5
|
| |
Toshima, T
|
209,000
|
8
|
27,000
|
1
|
| |
Ina, S
|
180,000
|
0
|
20,000
|
0
|
| |
Shiki, S
|
113,000
|
0
|
13,000
|
0
|
| |
Higashimurayama, T
|
28,000
|
0
|
2,000
|
0
|
|
(B)
|
Seashore sands
| | | | |
| |
Tateyama, C (20 m from sea)
|
282
|
6
|
64
|
6
|
| |
Tateyama, C (20 m from sea)
|
99
|
0
|
17
|
0
|
| |
Tateyama, C (5 m from sea)
|
501
|
13
|
24
|
4
|
| |
Tateyama, C (5 m from sea)
|
381
|
7
|
93
|
1
|
| |
Tateyama, C (0 m from sea)
|
244
|
12
|
8
|
1
|
| |
Tateyama, C (0 m from sea)
|
289
|
5
|
34
|
4
|
- For the determination of numbers of c.f.u., six inland and six seashore samples (0.5 g) were suspended in distilled water or 10% NaCl solution (2.0 ml), and serially dilutions were spread on agar media (0.1 ml/plate) with no or 20% NaCl, and incubated at 37°C. Numbers of endospore forming bacteria, were determined after heat treatment of the soil suspension at 80°C for 60 min. Numbers are averages of three experiments.